MLVA-typing on Streptococcus pneumoniae serotype 1 isolated from meningitis cases in Niger before the introduction of PCV-13 revealed a low genetic diversity.

Trans R Soc Trop Med Hyg. 2015 May 1. pii: trv033. [Epub ahead of print]

MLVA-typing on Streptococcus pneumoniae serotype 1 isolated from meningitis cases in Niger before the introduction of PCV-13 revealed a low genetic diversity.

Alio Sanda A1, Granger Farbos A2, Hamidou AA1, Vonaesch P1, Jusot JF1, Koeck JL2, Collard JM3.

Author information

Abstract

BACKGROUND:

Serotype 1 was the most prevalent pneumococcal meningitis serotype encountered in Niger over the period 2003-2011 (pre-vaccination era), accounting for 45.3% of infections.

METHODS:

Multiple locus VNTR analysis (MLVA) was used to create a genotypic snapshot of a representative subset of the pneumococcal population of serotype 1.

RESULTS:

MLVA using 16 markers revealed a homogeneous genetic background of pneumococci serotype 1 from Niger, which clustered with few serotype 1 pneumococci from some African countries, while other African countries displayed different clonal complexes. DNA from Niger and from other African countries were different from pneumococci serotype 1 from European countries.

CONCLUSIONS:

MLVA-typing revealed a low genetic diversity among pneumococci serotype 1 from meningitis cases in Niger in the pre-vaccination era.

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KEYWORDS:

Genetic diversity; Multiple locus VNTR analysis; Niger; Serotype 1; Streptococcus pneumoniae

PMID: 25934981 [PubMed - as supplied by publisher]