Streptococcus pneumoniae and influenza: dynamic changes in the pneumococcal transcriptome during transition from biofilm formation to invasive disease.

Infect Immun. 2014 Aug 18. pii: IAI.02225-14. [Epub ahead of print]

Streptococcus pneumoniae and influenza: dynamic changes in the pneumococcal transcriptome during transition from biofilm formation to invasive disease.

Pettigrew MM1, Marks LR2, Kong Y3, Gent JF4, Roche-Hakansson H2, Hakansson AP5.

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Abstract

Streptococcus pneumoniae is a leading cause of infectious disease globally. Nasopharyngeal colonization occurs in biofilms and precedes infection. Prior studies indicate that biofilm derived pneumococci are avirulent. However, influenza A virus (IAV) infection releases virulent pneumococci from biofilms in vitro and in vivo. Triggers of dispersal include IAV-induced changes in the nasopharynx such as increased temperature (fever) and extracellular ATP (tissue damage). We used RNA-seq to compare the S. pneumoniae transcriptome in biofilms, bacteria dispersed from biofilms after exposure to IAV, febrile-range temperature, or ATP, and planktonic cells grown at 37°C. Compared with biofilm bacteria, actively dispersed S. pneumoniae, which were more virulent in invasive disease, up-regulated genes involved in carbohydrate metabolism. Enzymatic assays for ATP and lactate production confirmed that dispersed pneumococci exhibited increased metabolism compared to those in biofilms. Dispersed pneumococci also up-regulated genes associated with production of bacteriocins and down-regulated colonization-associated genes related to competence, fratricide, and the transparent colony phenotype. IAV had the largest impact on the pneumococcal transcriptome. Similar transcriptional differences were also observed when actively dispersed bacteria were compared with avirulent planktonic bacteria. Our data demonstrate complex changes in the pneumococcal transcriptome in response to IAV-induced changes in the environment. Our data suggest that disease is caused by pneumococci that are primed to move to tissue sites with altered nutrient availability and to protect themselves from the nasopharyngeal microflora and host immune response. These data help explain pneumococcal virulence after IAV infection and have important implications for studies of S. pneumoniae pathogenesis.

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PMID: 25135685 [PubMed - as supplied by publisher]