Biotechnol Appl Biochem. 2014 Sep 4. doi: 10.1002/bab.1286. [Epub ahead of print]
Effects of recombinant IL-17F intranasal inoculation against Streptococcus pneumoniae infection in a murine model.
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Abstract
Interleukin (IL)-17F is an important member of IL-17 cytokine family, which plays important roles in host defense against microbial infections. Streptococcus pneumoniae (S. pneumoniae) is a common pathogen associated with several invasive and non-invasive pneumococcal diseases, and mucosal immune response plays crucial roles in defenses against pneumococcal infection. Thus, intranasal inoculation may be an alternative approach against pneumococci. In this study, BALB/c mice were intranasally inoculated with recombinant IL-17F (rIL-17F) prior to S. pneumoniae (ATCC 6303, serotype 3) infection. Compared to control group, the numbers of total leukocyte, neutrophil and macrophage in lung were significantly increased in mice inoculated with rIL-17F. The levels of macrophage inflammatory protein (MIP)-1α, MIP-2β and interferon (IFN)-γ were significantly increased in bronchoalveolar lavage fluid (BALF) and culture supernatant of splenocytes from mice inoculated with rIL-17F. rIL-17F inoculation also significantly elevated β-defensin-2 (Defb2) expression in lung tissue. Furthermore, compared to S. pneumoniae infection group, rIL-17F inoculation prior to infection significantly reduced S. pneumoniae colonization in lung. These findings demonstrated that rIL-17F intranasal inoculation strengthened host defense against pneumococci, which may be developed to prevent pneumococcal infection. This article is protected by copyright. All rights reserved.
Copyright © 2014 International Union of Biochemistry and Molecular Biology, Inc.
KEYWORDS:
Streptococcus pneumoniae; inflammation; interleukin-17F; β-defensin-2
PMID: 25196250 [PubMed - as supplied by publisher]
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